My experience is that when fixed brain stays long in PBS, it becomes very
soft. Than I don't use these brains anymore.
Reason? I don't know. Somebody else maybe?

Frouwke Kuijpers

----- Original Message ----- 
From: 
To: 
Sent: Tuesday, September 23, 2003 1:36 PM
Subject: [Histonet] Re: Brain Tissue in Buffer


>
> Hi,
> I've been told that if you fix in PFA then store in PBS for long periods
> the PBS "unfixes" the tissue. So I would suggest redoing your fixation
> process.
>
> Good luck!
>
>
>
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