Re: [Histonet] Paraffin Embedding of Mice Tissue

From:" Katri Tuomala"

Hi Kelly,

What is your fixative for these mice tissues and how long? How big (read
thick) are your sections to be processed?
These two things will determine your routine processing protocol.
I'm not familiar with microwave processing, so I won't comment on that.
If the tissue is well fixed, there really isn't a chance to "over process"
it. The problems arise with inadequate fixation, which then leads to
alcohols and xylene drying out the tissue, and hot paraffin then causes
further damage.
Just something to think about....


Katri Tuomala
Anatomic Pathology
St.Joseph's Health Care
Hamilton, Ontaorio, Canada

----- Original Message ----- 
From: "Kelly Salceies" 
Sent: Monday, September 29, 2003 10:01 AM
Subject: [Histonet] Paraffin Embedding of Mice Tissue

> Hi Everyone,
> I am a brand new Tech and am having trouble preping some mouse tissue
> for paraffin embedding. I have all different tissues (liver, heart,
> quads, brain, lung, and gastroc) and would like to prep all tissues (if
> possible)  under the same conditions. I have been using the Shandon
> Hypercenter XP or the Shandon Histowave (microwave tissue processor...).
> Does anybody have a good protocol for mouse tissues on either of these
> instruments?? I have tried a number of protocols, but all my tissue has
> been really dry in cutting...
> Any suggestions would be greatly appreciated!!
> Thanks,
> Kelly Salceies
> University of New Mexico
> Health Sciences Center
> Dept. of Pathology
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