Re: [Histonet] Opinion on storing sections on slides

From:Geoff McAuliffe

Hi Gareth:

    I would keep them in buffer in the refrigerator until I needed them. I have done immunos for tyrosine hydroylase and GFAP on sections stored at minus 80 C for several months. The GFAP was ok but the TH suffered from storage. Storage for just a few days at room temp ruined Mac1 for mouse microglia. Once sections dry out the immuno seems to go downhill.

Geoff

Davis, Gareth wrote:
 
Hello All,
I would like to get some input on storing sections.  I have cut frozen (PFA perfused and post fixated) tissue on a sliding microtome, kept the cut sections in PBS until mounting on slides.  Then I applied the sections to a SuperFrost Plus slide with water and dried down on a slide warmer-overnight.  So, the staining has turned out okay, but I wonder if I should keep the sections I don't use in the freezer, like typical frozen sections, or keep them at room temperature, like paraffin sections (since they are dry and already fixed).  I was going to try to stain free floating sections, but it just didn't work.=A0 So, any suggestions would be greatly appreciated.
Thanks,
Gareth
 
Ms. Gareth B. Davis
Research Assistant II
Neuro-magnetics Division of
the Department of Neurology
Vanderbilt University Medical Center
615-936-3318
 
 

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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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