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Hello All,
I would like to get some input on storing sections. I have cut
frozen (PFA perfused and post fixated) tissue on a sliding microtome,
kept the cut sections in PBS until mounting on slides. Then I applied
the sections to a SuperFrost Plus slide with water and dried down on a
slide warmer-overnight. So, the staining has turned out okay, but I
wonder if I should keep the sections I don't use in the freezer, like
typical frozen sections, or keep them at room temperature, like
paraffin sections (since they are dry and already fixed). I was going
to try to stain free floating sections, but it just didn't work.Â=A0 So,
any suggestions would be greatly appreciated.
Thanks,
Gareth
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Ms. Gareth B. Davis
Research
Assistant II
Neuro-magnetics
Division of
the Department of
Neurology
Vanderbilt
University Medical Center
615-936-3318
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