Re: [Histonet] H&E staining on GFP slides

From:Gayle Callis

WE quenched out GFP, although not eGFP with hematoxylin and Eosin will
fluorescse.  It is best to view GFP without trying to counterstain. 

Maybe some people have had success, but we did not. 

At 11:10 PM 9/23/2003 +0800, you wrote:
>
>
>Hi all,
>
>I want to do H&E staining on GFP expressing slides. So I can view both the
tructure of my slide and also GFP expressing. Is that possibile? If not,
what should I do?
>
>
>Baowei Peng
>Shanghai JiaoTong University,
>Shanghai,
>China,PRC
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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