Re: [Histonet] Fw: Brain Tissue in Buffer

From:Geoff McAuliffe

What exactly is the problem? If the tissue was properly fixed it should cut fine, no matter if storage was one week or one year (assuming no mold growth). Are you trying to embed in wax or cut frozen sections?


Mary Parker wrote:
----- Original Message -----
From: Mary Parker
Sent: Monday, September 22, 2003 8:03 AM
Subject: Brain Tissue in Buffer

If anyone has any experience about how to successfully process and microtome serial sections of rat brain tissue that was perfused, then stored in buffer solution for approx one year in the refrigerator...please help! We were given this tissue by an outside investigator and are having a miserable time of it.
I need all the information I can gather about tissue stored in buffer for an extended period of time.
Is it hopeless?
Mary Parker, H.T., A.S.C.P.
Histology Manager
EPL, Inc.
P.O. Box 12766
RTP, N.C. 27709
(919) 998-9407

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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