Re: [Histonet] COX-2 antibody
I am no expert but I have a little experience. In human tissues I had
to use heat retreival on my formalin-fixed, paraffin-embedded
tissues as well as leaving the antibody on overnight at 4 C. In
canine tissues I couldn't get it to work at all (although someone else
at this institution could, but I don't know the details), and in rats it
worked like a charm with ag retreival not required and incubation
for only 1 hr at room temperature! There are many different COX-2
ab's out there and as many or even more different sources. I think
the trick might be to find the one that suits your application best.
Date sent: Thu, 25 Sep 2003 07:28:13 -0500
From: "Goeden, Catherine"
Subject: [Histonet] COX-2 antibody
> I am working on a research project and wondering if anyone is using this
> antibody and could give me some information on it. I purchased the kit and
> antibody from Biocare Medical. They have told me it is sometimes tricky to
> use so if someone has some hints I would greatly appreciate it. Thanks in
> Histonet mailing list
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