Re: [Histonet] Alizarin red/Alcian blue double skeletal stain

From:John Kiernan

There's this method:

Webb GN & Byrd RA 1994. simultaneous differential
staining of cartilage and bone in rodent fetuses: an
alcian blue and alizarin red S procedure without
glacial acetic acid. Biotechnic & Histochemistry

The colour photo looks excellent. The instructions take
up 2 printed pages so more is needed than a simple
"protocol." The journal is available in all good
medical libraries. The method could probably be
simplified for chick embryos because they are smaller
than rat fetuses.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
"Grant, Debra" wrote:
> Does anyone have a protocol for Alizarin Red/Alcian Blue on chick embryo? I have several protocols for mouse. I have tried to adjust times in solution, but I have some problems with the cartilage staining in some of the chick embryos.
> Thank you in advance.
> Debby Grant
> Research Technician II
> Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th Street
> Kansas City, MO 64110

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