Re: [Histonet] AEC substrate for IHC

From:"John C. Dennis"

Dear Baowei Peng

Use an aqueous mountant or, depending on your substrate,  dehydrate in
ethanol and air dry.  Then mount
without using the clearing agent.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Sun, 21 Sep 2003, Baowei Peng wrote:

> hi, all histoneters,
>
> We can use ethanol to dehydrate and xylene to clear slides if we used DAB as substrate in IHC. Since AEC is organically soluble, we can not use these reagents.
> I am wondering what reagents we should use and should we dehydrate and clear AEC substrate slides?
>
> Baowei Peng
> Shanghai JiaoTong University,
> Shanghai,
> China,PRC
>
>
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