Re: FW: [Histonet] Gimenez stain

From:"Mary North"

Sigma has Alcian Yellow listed in the catalog # A 7394. American Master*Tech Scientific also has it listed.
Mary North, HT(ASCP,HTL
Oregon Health & Science University
Portland, OR
 


>>> "Demarinis,Carolyn" <cdemarinis@SARATOGACARE.ORG> 09/25/03 04:27AM >>>

We were using the Alcian Yellow Toluidine blue method for years, but
unfortunately
Alcian Yellow is not available in this country.  (I cannot remember the
problem). 
Therefore I have to find an alternative stain for H. Pylori.  I have
reordered a
Gimenez kit hoping that the kit I am using is a bad lot.  If Alcian Yellow
is=20
available for purchase, please let me know.  Thanks for your help.
Carolyn DeMarinis
Saratoga Hospital
Saratoga Springs New York

Have you tried the Alcian Yellow Toluidine blue method. I've tried most
methods over the years and this one, to me at least, is the best.
I can send you a protocol if you like.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of
Demarinis,Carolyn
Sent: 24 September 2003 18:36
To: HISTONET (E-mail)
Subject: [Histonet] Gimenez stain


We use the Gimenez stain for H. Pylori and until now it has worked
beautifully.  Unfortunately, the bugs are not staining
red, they are staining a very weak blue-green.  Can anyone help?
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