Any reason why you are not using "Aniline" blue stain? If it is not
available you can substitute Light green for the Aniline blue.

HM2(FMF) Douglas D. Deltour
Naval Hospital Sigonella Italy
LPO Histology
Histology Technician
DSN 624-4669
FAX 624-4680
COMM (From US) 011-39-095-56-4669


-----Original Message-----
From: Myri37@aol.com [mailto:Myri37@aol.com]
Sent: Friday, September 19, 2003 12:08 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] (no subject)


hi
i use Masson trichrome stain since a long time for paraffin sections, and i
don't know why now collagen doesn't stain in blue,  i make my  blue anilin
with methyl blue of SIGMA in water and  acetic acid, do you know any reason
that makes collagen does'tn stain in blue ? 
thank you very much for any help
Myriam

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