RE: [Histonet] apoptosis marker profile of gliomas



We have used with great success both an active caspase-3 and -9 from
Biovision. Our controls have been high grade gliomas. We retrieve with EDTA
and use at a dilution of 1/500 with an overnight incubation. If you require
details please let me know.


Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
Disclaimer: Not this little black duck!

-----Original Message-----
[]On Behalf Of Stylli,
Sent: Monday, 29 September 2003 04:30 PM
Subject: [Histonet] apoptosis marker profile of gliomas
Importance: High

Hello again Histonetters,
I will be looking at the apoptotic profile of FFPE human brain
tumours (various grades - low to high).
I wish to correlate the expression of various apoptotic markers to
the survival of the patients after certain treatments they have
been subjected to against a control group that have had surgery alone.
I have read a couple of papers that have used a few antibodies but
I would like to hear from people who have had success with
antibodies (with and without antigen retrieval).
As you can imagine, you can trial antibodies from different
companies until the cows come home and the bank is empty
thankyou for your help
Stan Stylli

Stan Stylli
Department of Surgery,
5th Floor Clinical Sciences Building
Royal Melbourne Hospital
University of Melbourne
Parkville, Australia, 3052.
Tel: 61-3-93427616

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