RE: [Histonet] antigen retrieval buffers

From:Luis Chiriboga

We make up 20 liter carboy of citrate, pH it let it sit at RT.   Most of the
time it doesn't sit around long enough to have any problems. If is a slow
time, add a crystal of Thymol. Same with EDTA.


-----Original Message-----
[]On Behalf Of Marion
Sent: Monday, September 29, 2003 9:59 AM
To: 'Stylli, Stanley'
Cc: Histonet (E-mail)
Subject: RE: [Histonet] antigen retrieval buffers

We make up 5 litres of 0.01M tri-sodium citrate buffer at a time and keep it
at room temperature. We decant what we need and pH it. The rest seems to
keep for a few weeks without any problem.

Bob Quilty
Dept of Neuropathology
Frenchay Hospital
Bristol, UK

-----Original Message-----
From: Stylli, Stanley []
Sent: 29 September 2003 06:50
Subject: [Histonet] antigen retrieval buffers
Importance: High

Hi Histonetters
I have a question about the longevity of antigen retrieval buffers.
If you make up your own buffers such as the following :
1 mM EDTA (pH 8.0)
10 mM Citrate (pH 6.0)
10 mM tri-sodium citrate (pH 6.0)
100 mM Tris / 1 mM EDTA (pH 9.0)
10 mM Tris (pH 10.0)
How long can these solutions be kept at 4C without affecting the efficiency
of the buffer.

Stan Stylli
Department of Surgery,
5th Floor Clinical Sciences Building
Royal Melbourne Hospital
University of Melbourne
Parkville, Australia, 3052.
Tel: 61-3-93427616

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