RE: [Histonet] antigen retrieval buffers
We make up 5 litres of 0.01M tri-sodium citrate buffer at a time and keep it
at room temperature. We decant what we need and pH it. The rest seems to
keep for a few weeks without any problem.
Dept of Neuropathology
From: Stylli, Stanley [mailto:Stanley.Stylli@mh.org.au]
Sent: 29 September 2003 06:50
Subject: [Histonet] antigen retrieval buffers
I have a question about the longevity of antigen retrieval buffers.
If you make up your own buffers such as the following :
1 mM EDTA (pH 8.0)
10 mM Citrate (pH 6.0)
10 mM tri-sodium citrate (pH 6.0)
100 mM Tris / 1 mM EDTA (pH 9.0)
10 mM Tris (pH 10.0)
How long can these solutions be kept at 4C without affecting the efficiency
of the buffer.
Department of Surgery,
5th Floor Clinical Sciences Building
Royal Melbourne Hospital
University of Melbourne
Parkville, Australia, 3052.
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