RE: [Histonet] Ret IHC

From:"Houston, Ronnie"


We were going to set this up a few months ago, and then had a new
pathologist, Lisa Cerilli, join us from UVA. She had studied and compared
Ret to CK19 for follicular thyroid lesions and came out in favor of CK19.
They did use the monolconal from Novocastra though.

Here is the abstract of her paper:

Am J Clin Pathol. 2002 Aug;118(2):186-93.
Interpretation of RET immunostaining in follicular lesions of the thyroid.
Cerilli LA, Mills SE, Rumpel CA, Dudley TH, Moskaluk CA.

Robert E Fechner Laboratory of Surgical Pathology, University of Virginia
Health Sciences Center, Charlottesville, USA.

We applied monoclonal antibodies against RET and cytokeratin 19 (CK19) to
the following tumor sections: classic papillary carcinoma (PC), 16;
Hurthle-type PC (HPC), 1; sclerosing PC with nodular fasciitis-like stroma
(SPC), 1; PC, follicular variant (FVPC), 12; follicular adenoma (FA), 9;
Hurthle cell adenoma (HA), 4; Hurthle cell carcinoma (HC), 3; and follicular
carcinoma (FC), 7. CK19+ tumors included 16 PCs, 1HPC, 1SPC, 11 FVPCs, 7
FAs, 4 FCs, and 1HC. RET+ tumors included 4 HAs, 3 HCs, 1HPC, 12 PCs, 7
FVPCs, and 2 FAs. Reverse transcriptase-polymerase chain reaction (RT-PCR)
revealed a RET transcript in 6 Hurthle cell lesions. RET immunoreactivity is
less sensitive and specific for PC than CK19. CK19 is useful for identifying
PC, although only lesions with diffuse, intense staining should be
considered positive. The detection of RET protein by immunohistochemical
analysis was corroborated by the presence of the RET transcript by RT-PCR.
Further study is warranted to determine whether this represents activation
by gene fusion or some other mechanism in this subset of thyroid neoplasms.

I would be interested in your results though.

Best wishes


Ronnie Houston 
Regional Histology Operations Manager 
Bon Secours HealthPartners Laboratories 
5801 Bremo Road 
Richmond, VA 23226 
(804) 287 7972 

-----Original Message-----
From: Richard Cartun []
Sent: Wednesday, September 17, 2003 12:25 PM
Subject: [Histonet] Ret IHC

We are do "Ret" immunoperoxidase staining on thryoid tissue using a
rabbit polyclonal antibody from Santa Cruz (sc-167).  Does anyone have
experience with this antibody on formalin-fixed, paraffin-embedded
tissue?  Thank you.

Richard Cartun

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