RE: [Histonet] Purpose of Oven Prior to Staining?

From:"Kemlo Rogerson"

5. To create artifacts.

Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS
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-----Original Message-----
[] On Behalf Of Tony
Sent: 19 September 2003 00:00
Cc: 'Jordan Brod'
Subject: RE: [Histonet] Purpose of Oven Prior to Staining?


Reasons for drying slides above the melting point of wax:
1.	Remove excess water
2.	Allow sections to attach to slides (otherwise they will lift off
the dewaxing xylene).
3.	Aid in final spreading out of sections so that there are fewer
(should I say NO folds!!)
4.	Remove some of the excess wax (ie it flows off the slide).
this requires regular cleaning of the oven to remove excess wax.
5.	Can't think of any other reasons

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Jordan Brod []
Sent: Friday, 19 September 2003 0:41
Subject: [Histonet] Purpose of Oven Prior to Staining?

Good Morning All:

My lab is in a huge discussion about the reason why we put our slides in
oven before staining.  I told them that the reason was to dry all of the
water off of them, because xylene and water do not mix.  This statement
also backed up in literature that I have read.  They still do not
me, because they think the purpose of the oven is to deparaffinize the

Can anyone help me out, so I can put this discussion to rest?  I will
off all of your comments for my technicians to read.  Is what I told
correct or not?



Jordan W. Brod
Diagnostic Lab Supervisor - Pathology
Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
1 Sippel Road
TAMU 4471
College Station, Texas 77843
(979) 845-3414
(979) 845-1794 fax

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