RE: [Histonet] Microtome for autoradiography
You had best look into this a bit deeper. The microtome you are planning
to purchase is not large enough for sectioning whole rats unless they
are only 130mm in length.
For WBA section you require a cryostat not a bench microtome unless you
would like to place the microtome in a -20șC cold room. I am not joking
this is why we started to manufacture this type of cryostat, as Bob
McCulloch at Glaxo, Fulmer Hall, England, 40 years+ ago was doing so, as
he could only stay at this temperature for 1/2 hour max. he asked us to
produce a large sectioning cryostat for him, we did and many 100's were
sold for WBA.
A Tungsten Carbide Knife will have no problems sectioning through the
We manufacture a range of cryostats for Whole Body Autoradiography
This is an outline of the process for WBA sectioning.
The appropriate safety equipment must be used e.g. eye protection and
1) Pin the specimen by the limbs onto a flat 3mm or 1/8" sheet of
2) Immerse the above into a slurry of Petroleum Ether and Solid C02
solution, for at least 4 hours..
3) Remove from hardboard and remove limbs and tail.
4) Wash fur with a 0.07% solution of Trinton X.
5) Immerse specimen into a mould and fill with a 2% solution of Carboxy
6) Finally immerse the above into a slurry of Petroleum Ether and Solid
C02 solution, for at least 2 hours.
7) Remove from mould and mount the moulded specimen onto a object holder
for mounting onto the microtome using a 2% solution of Carboxy Methyl
8) Using a Tungsten tipped knife trim specimen until the required level
is reached, adhere 3m's or equivalent section mounting tape to the face
of the sample and cut the required section @ 20-30” depending on
I hope you find this useful.
Bright Instrument Co.Ltd.
St Margaret's Way
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site: www.brightinstruments.com
From: gamal akabani hneide [mailto:email@example.com]
Sent: 29 September 2003 05:02
Subject: [Histonet] Microtome for autoradiography
Hi All - I need some advice regarding the best microtome to use for
body autoradiography for rats. I am planning to buy a sledge microtome
SM2400 from Leica.
I am going to inject a radiolabeled MAB and sacrifice the animal with
minutes to hours, what next? What about the bone sections? Etc.
Any references will be appreciated. Thanks in advanced
Gamal Akabani, Ph.D.
Duke University Medical Center
Department of Radiology
Division of Nuclear Medicine
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