RE: [Histonet] Fw: Brain Tissue in Buffer

From:"Favara, Cynthia (NIH/NIAID)"

I had some investigators that insisted on putting mouse brain in PBS @4C to insure fixation was for a uniform time. Sometimes these were in PBS[sterile] for months. When I got them ran them through the processor with and initial NBF station. I don't recall them being any different unless the fixation was inadequate.
Good Luck!

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Mary Parker []
Sent: Monday, September 22, 2003 6:05 AM
Subject: [Histonet] Fw: Brain Tissue in Buffer

----- Original Message -----
From: Mary Parker
Sent: Monday, September 22, 2003 8:03 AM
Subject: Brain Tissue in Buffer

If anyone has any experience about how to successfully process and microtome serial sections of rat brain tissue that was perfused, then stored in buffer solution for approx one year in the refrigerator...please help! We were given this tissue by an outside investigator and are having a miserable time of it.
I need all the information I can gather about tissue stored in buffer for an extended period of time.
Is it hopeless?
Mary Parker, H.T., A.S.C.P.
Histology Manager
EPL, Inc.
P.O. Box 12766
RTP, N.C. 27709
(919) 998-9407

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