RE: [Histonet] FLI-1

From:"Houston, Ronnie"

Hi Liz,

FLK-1 and FLT-1 are cell membrane receptors for VEGF; Fli-1 is a transcription factor/regulator. As it is a nuclear protein, yes, in a strict sense, it doesn't stain the same as CD31 or CD34 or Factor VIII, but it is specific for endothelial cells.

Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972

Hi Liz,

-----Original Message-----
From: Elizabeth Chlipala []
Sent: Thursday, September 25, 2003 10:58 AM
To: 'Fearn Tony';
Subject: RE: [Histonet] FLI-1


I believe the antibody you are talking about is FLK-1 or FLT-1, another
name for it is VEGF-R2.  From what I understand it does not stain the
same as CD34, I don't believe it is specific for endothelial cells, it
will stain other things also.  You can get this antibody from Santa
Cruz.  We have worked on this in the past and in our hands it was a
tough one.  It does play a role in angiogenesis.


Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540

Ship to Address:
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University of Colorado
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-----Original Message-----
[] On Behalf Of Fearn Tony
Sent: Thursday, September 25, 2003 5:15 AM
To: ''
Subject: [Histonet] FLI-1

> One of our pathologists has just returned from a conference in Milan
> is asking if we can get FLI-1, as an endothelial marker, that is meant
> be better than CD34. I've looked in the DakoCytomation and Novocastra
> catalogues but there is no mention of it. Does anyone know where I can
> it, and is it worth buying?
> Anthony J Fearn
> Chief Biomedical Scientist
> Histopathology

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