From: | "Houston, Ronnie" |
Hi Liz,
FLK-1 and FLT-1 are cell membrane receptors for
VEGF; Fli-1 is a transcription factor/regulator. As it is a nuclear protein,
yes, in a strict sense, it doesn't stain the same as CD31 or CD34 or Factor
VIII, but it is specific for endothelial cells.
Ronnie
Ronnie Houston
Regional Histology Operations
Manager
Bon Secours
HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804)
287 7972
ronnie_houston@bshsi.com
Hi Liz,
-----Original Message-----
From: Elizabeth Chlipala [mailto:lizchlipala@premierhistology.com]
Sent:
Thursday, September 25, 2003 10:58 AM
To: 'Fearn Tony';
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet]
FLI-1
Tony
I believe the antibody you are talking about is
FLK-1 or FLT-1, another
name for it is VEGF-R2. From what I understand
it does not stain the
same as CD34, I don't believe it is specific for
endothelial cells, it
will stain other things also. You can get this
antibody from Santa
Cruz. We have worked on this in the past and in our
hands it was a
tough one. It does play a role in
angiogenesis.
Liz
Elizabeth A. Chlipala, BS,
HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder,
Colorado 80308
Office: (303) 735-5001
Fax: (303)
735-3540
lizchlipala@premierhistology.com
www.premierhistology.com
Ship
to Address:
Premier Histology Laboratory
University of Colorado
MCBD,
Room A3B40
Boulder, Colorado
80309
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-----Original Message-----
From:
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]
On Behalf Of Fearn Tony
Sent: Thursday, September 25, 2003 5:15 AM
To:
'histonet@lists.utsouthwestern.edu'
Subject: [Histonet]
FLI-1
> One of our pathologists has just returned from a
conference in Milan
and
> is asking if we can get FLI-1, as an
endothelial marker, that is meant
to
> be better than CD34. I've looked
in the DakoCytomation and Novocastra
> catalogues but there is no mention
of it. Does anyone know where I can
get
> it, and is it worth
buying?
>
> Anthony J Fearn
> Chief Biomedical
Scientist
>
Histopathology
>
>
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