[Histonet] isopropanol in processing

From:Steven Slap

Hi HistoNetters

As John Kiernan pointed out, isopropanol is not miscible with molten
paraffin.  In microwave processing, the paraffin is heated above the boiling
point of the isopropanol (in some instruments, under vacuum, allowing lower
temperatures to be employed), and the remaining isopropanol is "flash
evaporated" out of the tissue.

Steven Slap

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