Dear all,

Can anyone help me? I am trying to visualise Kupffer cell subpopulations using ED1 and ED2. ED1 is working fantastically well but I am getting nothing with ED2. Both primaries are from Serotec Oxford. I have tried both unmasking and not unmasking by autoclave. I am using a DAKO strepABC visualisation kit with DAB substrate. I would be indebted to anyone who could offer me some advice.

Damian Mole
Queen’s University Belfast
Northern Ireland


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