Re: [Histonet] validating oil red O
From: | Paul Howard Lockwood |
Dear Jeremy,
I would suggest using frozen sections, and mounting them on slides coated with poly-l lysine. Also, do not expose the slides to heat above room tempeture.
I hope this is of some help to you.
Sincerely,
Paul Lockwood
9/11/03 6:53:05 PM, Jeremy Browne wrote:
>
>
>
> We need to set up a range of fat concentrations on slides, stain and then
> check to see that there is a linear response relationship between fat
> concentration and staining intensity but because fat melts and slides off
> histoworld has solved this problem - perhaps with an intermediatere is
> substance to which the fat adheres?
> Thanks
> Jeremy Browne
> Liggins Institute
> NZ (09) 373 7599 extn 86444
> j.browne@auckland.ac.nz
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>