----- Original Message -----

From: Jeremy Browne

To: 'histonet@lists.utsouthwestern.edu'

Sent: Friday, September 12, 2003 3:53 AM

Subject: [Histonet] validating oil red O

Anyone out there with an idea of oil red O staining could be validated?

We need to set up a range of fat concentrations on slides, stain and then check to see that there is a linear response relationship between fat concentration and staining intensity but because fat melts and slides off slides this is not easy.  However, I imagine that someone out there is histoworld has solved this problem - perhaps with an intermediate substance to which the fat adheres?

Thanks

 

Jeremy Browne

Liggins Institute

University of Auckland

NZ (09) 373 7599 extn 86444

j.browne@auckland.ac.nz