Re: [Histonet] how to embed/section mesentery?

From:Geoff McAuliffe

Hi Felix:

    For mesentaries some people like a "spread" rather than a section. 
Take out several loops of intestine and pin them out on a sheet of cork 
or dental wax with a tiny bit of tension on the mesentary so it is flat. 
Submerge in fix. You can process the whole "block" this way throught 
alcohols and clearing, then cut out and mount the sheet of mesentary on 
a slide without embedding. Yes it will be thicker than a section but you 
will see a nice 3D tissue with capillaries etc.

Geoff wrote:

>Dear all,
>Is there anybody who may give me advice how to make good sections of
>parafin embedded mesentery (mouse)? How to prepare the tissue for embedding
>and how to handle it to embed/orient it properly in the parafin block? My
>concern is especially the soft consistency of the tissue making me feel
>that the proper orientation in the block might be difficult.
>Thank you very much in advance,
>Felix Rintelen
>Serono Pharmaceutical Research Institute
>Geneva, Switzerland
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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