Stanley.Lupo@GSK.Com
Safety Assessment
Mail Code: UE0461
Phone: 610 270-7340
Fax: 610 270-7202
Fellow Histonetter,
In our lab, proper orientation begins at prosection. The mesentery is laid out flat, then rolled around the rubber plunger of a 1 mL syringe. The button that is formed when the rolled mesentery is removed from the plunger is placed in a plastic capsule and fixed in 10% Neutral Buffered Formalin.
Processing involves 40 minute cycles through 95% EtOH, then 60 minute cycles through 100% EtOH, 40 minutes each through 2 changes of toluene, followed by 4 changes of paraffin at 150 minutes (total). Total processing time is 9 hours.
The mesenteric roll is embedded on edge, and care is taken not to oversoak the block.
Good luck.
Stan Lupo
Department of Safety Assessment
GlaxoSmithKline
Felix.Rintelen@serono.com
Sent by: histonet-admin@lists.utsouthwestern.edu
18-Sep-2003 04:18
To: Histonet
cc:
Subject: [Histonet] how to embed/section mesentery?
Dear all,
Is there anybody who may give me advice how to make good sections of
parafin embedded mesentery (mouse)? How to prepare the tissue for embedding
and how to handle it to embed/orient it properly in the parafin block? My
concern is especially the soft consistency of the tissue making me feel
that the proper orientation in the block might be difficult.
Thank you very much in advance,
Felix
Felix Rintelen
Serono Pharmaceutical Research Institute
Geneva, Switzerland
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