If the smears are quite thick and bloody, you may want to use a more
dilute hydrogen peroxide solution (1% or so) for quenching.  The
vigorous reaction can strip material from the slide.  Prolonged buffer
wahes are a good idea as well.

Fred

>>>  09/16/03 08:30AM >>>
A colleague of mine would like to stain murine blood smears for Bcl2.
Does 
anyone have any experience doing IHC on blood smears which have been
fixed 
in Methanol? 

Thanks

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
847.938.4919
Jackie.O'Connor@abbott.com

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