Re: [Histonet] Finger Nail Softening
10% KOH soften nail-tissue. We let the tissue in
the solution for a few hours.
Burck writes: tissue should be fixed in formalin,
Alk. till 70%, than treat with "Chlordioxydessigsäure" (=Diaphenol,
=Chloratren from Chroma). This solution bleach Melanin.
I hope this helps.
Gundi Lang
general hospital, Linz, Austria
----- Original Message -----
Sent: Thursday, September 18, 2003 4:18
PM
Subject: [Histonet] Finger Nail
Softening
Hey all!
I was asked a question about softening finger nails before or during
processing that would make them easier to cut and allow them to hold on to the
slide. They want to do this instead of any kind of treatment to the nails
after they are embedded in paraffin so that it isn't just on the surface. If
anyone has a procedure for this or a reference from a manual I would really
appreciate it. You can email me directly at
banorris@utmb.edu
thanks once again
Brent A. Norris
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