RE: [Histonet] how to embed/section mesentery?


Hi Felix,

I guess it depends on what you are looking for in the mesentery.  Last
time we did this (in rats), we trimmed by starting with the mesenteric
lymph node, and trimmed all the mesentery off by following the GI tract.
Then, using the lymph node as a center point, spread out the mesentery
between 2 sponges and processed flat.  That way it comes out of the
processor ready to embed flat!

Hope this helps, 

Noelle Linke, BS, HTL(ASCP)
Allergan, Inc
Irvine, CA 92612

-----Original Message-----
From: [] 
Sent: Thursday, September 18, 2003 1:19 AM
Subject: [Histonet] how to embed/section mesentery?

Dear all,

Is there anybody who may give me advice how to make good sections of
parafin embedded mesentery (mouse)? How to prepare the tissue for
and how to handle it to embed/orient it properly in the parafin block?
concern is especially the soft consistency of the tissue making me feel
that the proper orientation in the block might be difficult.

Thank you very much in advance,


Felix Rintelen
Serono Pharmaceutical Research Institute
Geneva, Switzerland

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