RE: [Histonet] de-staining immunos

From:Tony Henwood

Amy,

Diff-quik slides can be destained. Place in 1%acetic acid in ethanol. This
will remove most of the dyes. 

As for IP stained slides, unless an ethanol soluble substrate is used (eg
AEC), it may be very difficult to remove the DAB substrate. You could try 5
min treatment with KMnO4 followed by Oxalic acid, though I can't be certain
this will work.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Amy Self [mailto:ASelf@gmhsc.com]
Sent: Wednesday, 10 September 2003 20:02
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] de-staining immunos


	
		
	Does anyone out in histoland know if Immuno stained slides and/or
air-dried Diff-Quik stained slides can be de-stained? If so could you please
share with me this procedure.  I know that H&E's can be de-stained but I
wasn't sure about immunos and diff-quiks.  Thanks for the help....


	Amy Self
	Georgetown Memorial Hospital
	Georgetown, SC
	(phone) 843-527-7179
	(fax) 843-520-7882


Note:  The information contained in this message may be privileged and
confidential and protected from disclosure. If the reader of this message is
not the intended recipient, or an employee or agent responsible for
delivering this message to the intended recipient, you are hereby notified
that any dissemination, distribution or copying of this communication is
strictly prohibited.  If you have received this communication in error,
please notify us immediately by replying to the message and deleting it from
your computer.  Thank you.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>