RE: [Histonet] TESTICULAR BIOSY

From:Tony Henwood

Beware,
 
Protein picrates are believed to be quite soluble in water. I would recommend storing in 80% or more ethanol prior to processing, not NBF. Fixation time 4-8 hours for a small biopsy (up to 4mm in diameter)
 

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Travis Troyer [mailto:ttroyer@pcllab.com]
Sent: Friday, 12 September 2003 23:07
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] TESTICULAR BIOSY

Our lab is receiving a testicular biopsy that will be received in Bouin's Solution.  We routinely place specimans in 10% NBF after grossing.  So, my question is how long should the biopsy stay in the Bouin's solution for proper fixation before placing into 10% NBF?
Thanks!


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