Travis,

In our lab we fix testicular biopsies in Bouin’s solution for 2-3 hours, wash in several changes of 70% alcohol to remove the picric acid and then place in 10% NBF for processing.  The removal of picric acid from the tissue is necessary to insure proper staining.

 

Lynne A. Bell, HT (ASCP)

Central Vermont Hospital

P. O. Box 547

Barre, VT  05641

802-371-4122