RE: [Histonet] Orcein Variation for EVG to detect Elastosis Perfo rans Serpiginosum

From:Tony Henwood

Kevin,
 
The following article may be of use:
 
Henwood, A., (2002) "The Orcein Stain - A Versatile Stain for Histopathology" J. Histotechnol 25(1):29.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: kevin williams [mailto:akwilliams75@hotmail.com]
Sent: Monday, 1 September 2003 4:15
To: MTitford@aol.com; histonet@pathology.swmed.edu
Subject: [Histonet] Orcein Variation for EVG to detect Elastosis Perforans Serpiginosum





Dear MT( and all you HTs out there):

Thanks for the information lots of help:

I am now on the quest for a procedure for Elastosis Perforans Serpiginosum.

My pathologist has asked for the Orcein variation on the EVG(Elastin Van Gieson) or VVG as some of you know it. I have to get the Orcein so that is my first challenge, second is my protocol. There are a few out there and I would like to here from anyone who has a favorite for this method with this disease process.

Yours faithfully A. Keivn Williams

>From: MTitford@aol.com
>To: Histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Alpha Amylase
>Date: Fri, 29 Aug 2003 15:50:51 -0400
>
>Robert Lotts asks about amylase:
>We use Sigma Alpha amylase Cat # A-3176.(0.125% at 37 degrees centigrade for 30 minutes). I zap it in the microwave to get it up to temperature
>
>Mike Titford
>USA Pathology
>Mobile AL 36617 USA
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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