RE: [Histonet] BrdU and EM?
Animal tissue (domestic chicks), transcardially perfused.
Subject: RE: [Histonet] BrdU and EM?
Date sent: Wed, 17 Sep 2003 08:03:03 -0500
From: "Charles W. Scouten, Ph.D."
To: "Mezey Szilvia"
> Animal tissue (perfused?) or human tissue?
> Charles W. Scouten, Ph.D.
> 5918 Evergreen Blvd.
> St. Louis, MO 63134
> Ph: 314 522 0300
> FAX 314 522 0377
> -----Original Message-----
> From: Mezey Szilvia [mailto:email@example.com]
> Sent: Wednesday, September 17, 2003 6:54 AM
> To: firstname.lastname@example.org
> Subject: [Histonet] BrdU and EM?
> Hello All,
> I'm trying to do BrdU staining (in paraformaldehyde fixed, free-
> floating sections) and preserve the ultrastructure of the tissue for
> EM at the same time. HCl works fine for denaturing DNA for BrdU-
> ICC but doesn't leave much of the tissue for EM. DNase would be
> more EM-friendly but doesn't penetrate the tissue enough.
> Does anybody know a method that could provide a fair enough
> compromise between BrdU and EM? Maybe by increasing the
> penetration of DNase?
> Best regards to you all,
> Szilvia Mezey
> PhD student
> Semmelweis University
> Dept. of Anatomy, Histology and Embryology
> Tuzolto u. 58. Budapest, 1094, Hungary
> T.: +36-12156920/3687
> F.: +36-12155158
> E-mail: email@example.com
> Histonet mailing list
Dept. of Anatomy, Histology and Embryology
Tuzolto u. 58. Budapest, 1094, Hungary
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