Robert,

A longlife solution (10min room temp incubation) is given in:

Mangan, V-M, Farago, V., Kelly, M., Henwood, A.F., (2002) "An Amylase
Reagent with a Long Shelf Life for the removal of Glycogen from Tissue
Sections" J. Histotechnol 25:153.

The amylase reagent used is as follows:
Alpha Amylase from Bacillus Subtilis (Fluka Cat No 10070,)	1g
	(59 U/mg Lot 12580/1 44301)
Oxoid PBS Tablets (Cat No BR14a)					1
tablet
Distilled water
100ml
Sodium Azide								0.1g

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Lott, Robert [mailto:Robert.Lott@bhsala.com]
Sent: Saturday, 30 August 2003 5:33
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alpha-amylase for PAS w/digestion


For those of you that use alpha-amylase... "exactly" what kind of reagent do
you order for this procedure?

Thanks!
Robert L. Lott, HTL(ASCP)
Manager, Anatomic Pathology
Baptist Health System
800 Montclair Road
Birmingham, AL  35213
205-592-5388|205-592-5646 (fax)
robert.lott@bhsala.com


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