Diana

How often you change solutions or stains on any stainer depends on the
amount of slides you are staining. It is never a good idea to top off or
add to a stain more than once. It is much better to completely change the
stain out whenever possible. If you keep on adding fresh stain to stain
already used, and never completely change the stain, your stain is always
diluted. It becomes a situation of "it is not really new and not it is not
really old", it is just diluted.

Sometimes small institutions only change stains once a week. In that
situation you will probably add some new stain to the old once a week. Just
make sure at the end of a week you throw the stain away and start with
fresh stain on Monday. It is always a good idea to run a couple of control
slides before you start a staining run everyday. Check your slides and see
how your stain really looks.

As far as filtering stains, it depends on the manufacturer. It also depends
on how long you use that stain on the stainer and how many slides you
stain.

If  have any questions just send me an email.


Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot@leica-microsystems.com
www.leica-microsystems.com



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