I usually fix all mouse tissues in either 10% formalin or 4%PFA for at 
least 24 hours. And then process on an automated tissue processor for a 
total of 4 hours using heat and vacumn. The process cycle used is as 
follows: #1 Wash in running tap H20 for 30 min. to remove fixative. #2  70% 
ETOH 40 min. #3  80% ETOH 10 min. #4  95% ETOH 10 min. #5 95% ETOH 10 min. 
#6  95% ETOH 20 min. #7  100% ETOH 20 min. #8 100% ETOH 20 min. #9 100% 20 
min. #10  Clearing agent 15 min. #11 Clearing agent 25 min. #12 Paraffin 20 
min. and #13 Paraffin 30 min. Best wishes. Atoska


Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 


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