[Histonet] Opinion on storing sections on slides

From:"Davis, Gareth"

Hello All,
I would like to get some input on storing sections.  I have cut frozen (PFA perfused and post fixated) tissue on a sliding microtome, kept the cut sections in PBS until mounting on slides.  Then I applied the sections to a SuperFrost Plus slide with water and dried down on a slide warmer-overnight.  So, the staining has turned out okay, but I wonder if I should keep the sections I don't use in the freezer, like typical frozen sections, or keep them at room temperature, like paraffin sections (since they are dry and already fixed).  I was going to try to stain free floating sections, but it just didn't work.  So, any suggestions would be greatly appreciated.
Ms. Gareth B. Davis
Research Assistant II
Neuro-magnetics Division of
the Department of Neurology
Vanderbilt University Medical Center

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