[Histonet] Muscarinic receptor detecting
Dear All,
I am currently trying to detect the existence of
different muscarinic receptor subtypes in human tissues using
immunofluorescence. My samples are OCT imbedded. I have tried
fixation with 4% paraformaldehyde, acetone, acetone:methanol, 95% ethanol,
neutral buffered formalin. I have used primary antibodies from R &
D and Biogenesis. So far all the antibodies light up the whole
cell with no intensified staining on the plasma membrane.
I wonder if any one has done stainings on
muscarinic receptors or other 7 transmembrane protein receptors and like to
share your success with me.
Thank you very much in advance.
Andea Liu
Ph D student
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