[Histonet] Masson Trichrome stain

From:

Brian Hatcher

I am attempting to use Masson's trichrome stain on some rat bone marrow stem cells cultured on fibrous contstructs. Following staining with the acid fuchsin, a large amount of tissue growing in between the fibers is staining red. Following the treatment with aniline blue, however, this tissue is no longer visible. In some areas it appears as though it has torn away from the fibers where it was previously spread between (some arease of floating tissue are visible). My initial thoughts were that perhaps this tissue was muscle, although this was a bit supprising as these cells should be differentiating into osteoblasts in the presence of these fibers. I had also read somewhere that the collagen would stain red with acid fuchsin, however, subsequent staining with aniline blue should result in collagen appearing blue. The only problem is I am seeing neither blue nor red cells in these specific areas following aniline blue staining. Any suggestions???
Thanks
Brian

--
template Brian Hatcher
Graduate Research Assistant
Department of Biomedical Engineering
University of Florida
PO Box 116400
Gainesville, FL 32611-6400
Ph: 352-392-6656
Fax: 352-392-3771
email: briany@ufl.edu

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