Phillipe,

You may want to consider trying either a different vendor or a different
antibody lot # at this point.  Once you get up to a 1:10 dilution, you
should be getting at least some staining somewhere...  Email me directly and
I'll give you some suggestions that I could catch heat for posting to the
general list.  gdawson@milw.dynacare.com

Good Luck,

Glen Dawson  BS, HT & QIHC (ASCP)
Lead IHC Technologist
Milwaukee, WI




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