[Histonet] Help! With LEICA TP1020 Automatic Tissue Processor
From: | hho@neurology.bsd.uchicago.edu |
Dear Histonetters,
Does anybody use LEICA TP1020 Automatic Tissue Processor? If do, do you have
a good tissue process protocol I could try out for Mice brain, spinal cord,
muscle...? I've been smashing my head on the wall to try to get a good
block for months, so far my head hurts. If you have any pointer or
suggestion please reply or e-mail me PLEASE!!! Thanks in advance =).
here's the protocol I've been using for LEICA TP1020 ATP.
70% ETOH 25min NO VAC
80% ETOH 25min NO VAC
95% ETOH 25min NO VAC
100%ETOH 25min NO VAC
100%ETOH 25min NO VAC
100%ETOH 25min NO VAC
Xylene 25min NO VAC
Xylene 25min NO VAC
Paraffin 45min (58 Degree) VAC
Paraffin 45min (58 Degree) VAC
Hanson
a very frustrated Lab tech.
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