[Histonet] BrdU and EM?
I'm trying to do BrdU staining (in paraformaldehyde fixed, free-
floating sections) and preserve the ultrastructure of the tissue for
EM at the same time. HCl works fine for denaturing DNA for BrdU-
ICC but doesn't leave much of the tissue for EM. DNase would be
more EM-friendly but doesn't penetrate the tissue enough.
Does anybody know a method that could provide a fair enough
compromise between BrdU and EM? Maybe by increasing the
penetration of DNase?
Best regards to you all,
Dept. of Anatomy, Histology and Embryology
Tuzolto u. 58. Budapest, 1094, Hungary
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