[Histonet] Auto stainer program schedules

From:Diana McCaig

We use a LEICA Autostainer XL and Surgipath Stains (Gil III haematoxylin
and Eosin).
The stains used to be filtered daily but it rendered the slides too dark so
the filtering was reduced to weekly.  I want to revert to daily filtering
but am getting some resistance.  We use 90 seconds in the haematoxylin
followed by 1 minute in Scott's Tap Water Substitute.  Can anyone share
their time schedule for routine H&E's.  Do you dilute your stains?  How
frequently are they changed or are they just topped up?  Thanks in advance
Diana McCaig, R.T. 
Charge Tech, Histology
Chatham Kent Health Alliance
519-352-6401 (3347)


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