toluidine blue/Na borate

Hey histonetters!!

I have a question or two.  What is the difference between 1%toluidine blue used 
alone and 1% toluidine blue added to 1% Na borate.  I know that toluidine blue 
is used on paraffin sections for mast cells.  I looked in histonet archives, 
but did not find an answer to my question.  Does anyone know why na borate is 
added? I know that 1% toluidine blue alone is dark blue with light blue;  add 
na borate and you get purple and blue.  

Any help appreciated!!


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