some general questions on the TUNEL assay

From:Kristel Wautier

Hello everybody,
we are planning to use the TUNEL assay for the detection of apoptosis on paraffin sections in the near future. However, we are new at this technique and we have some general questions:
First of all, is it necessary to work DNase free to perform this technique?
Secondly, the material we intend to use this technique on, needs to be decalcified. We usually use either Decalc (on the base of HCl) or EDTA. We wonder however, if these products could interfere with the TUNEL-detection. Does anybody now, for example, what the effect of EDTA or HCl on DNA is?
Kristel Wautier
Ghent University
Department of Biology
K.-L. Ledeganckstraat 35
9000 Ghent
Tel.: +32 9 264 52 31
Fax: +32 9 264 53 44

<< Previous Message | Next Message >>