some general questions on the TUNEL assay
we are planning to use the TUNEL assay for the
detection of apoptosis on paraffin sections in the near future. However, we are
new at this technique and we have some general questions:
First of all, is it necessary to work DNase free to
perform this technique?
Secondly, the material we intend to use this
technique on, needs to be decalcified. We usually use either Decalc (on the base
of HCl) or EDTA. We wonder however, if these products could interfere with the
TUNEL-detection. Does anybody now, for example, what the effect of EDTA or HCl
on DNA is?
K.-L. Ledeganckstraat 35
Tel.: +32 9 264 52 31
Fax: +32 9 264 53
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