peptides
Recently it has been suggested that in selecting an antibody to detect
an antigen in another species, a search for a matching peptide sequence
may be necessary. Specifically, we used a rabbit polyclonal antibody
raised to a 14-peptide chain from mouse iNOS, and obtained selective
staining with a reasonable pattern in the sheep. It was suggested that
since we could not find this a matching sequence in the sheep protein,
the antibody should not be used. I would greatly appreciate
the comments of the group on the usefulness of this kind of criterion.
Hal Hawkins, UTMB Galveston
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