paraffin embedding cells
Agreed re Cytospin. However we haven't,
unfortunately, got one. I gently scrape the cells from culture plate( can use
Trypsin BUT if the Ag is membrane-bound you will probably get a diminished
signal), then spin them down briefly to get a pellet. Then tip off the SC
snatant and add fixing fluid. Pellet usually comes away with gentle teasing
after fixing. Bit Heath-Robinson but gives adequate pellet for
sectioning
Carl Hobbs
Laboratory Manager
Molecular
Neurobiology Group
MRC Centre for Developmental Neurobiology
4th
Floor,South Wing
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