paraffin embedding cells

From:carl Hobbs

Agreed re Cytospin. However we haven't, unfortunately, got one. I gently scrape the cells from culture plate( can use Trypsin BUT if the Ag is membrane-bound you will probably get a diminished signal), then spin them down briefly to get a pellet. Then tip off the SC snatant and add fixing fluid. Pellet usually comes away with gentle teasing after fixing.  Bit Heath-Robinson but gives adequate pellet for sectioning
Carl Hobbs
Laboratory Manager
Molecular Neurobiology Group
MRC Centre for Developmental Neurobiology
4th Floor,South Wing
New Hunts House
Guy's campus
020 7848 6810

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