mounting media for double labeling
does anybody know of a mounting media compatible with double labeling
using the fluorescent cyanine dyes (CY2 & CY3) and BCIP/NBT. These
particular fluorescent dyes won't withstand the anti-fading agent
phenylendiamine; the BCIP/NBT is compromised by xylene-miscible
mountants like permount and cytoseal.
Any ideas?
Thanks,
Susan Travers
Susan Travers, Ph.D.
Associate Professor
Ohio State University
College of Dentistry
Section of Oral Biology
305 West 12th Avenue #192
P.O. Box 182357
Columbus, Ohio 43218-2357
Tel: (614)292-6366
Fax:614-247-6945
email: travers.3@osu.edu
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