decalcification of Carnoy or Methacarn fixated tissues

From:Kristel Wautier

I have a spin off question from my earlier postings on alcohol-based fixation for PCNA detection. Does anybody have suggestions on decalcifying using EDTA of these tissues fixated in Carnoy or Methacarn. We traditionally use 4% PFA or PG as fixative, we then decalcify with 0.1M EDTA in the fixative used.
However, this doesn't seem to work with alcohol-based fixatives (EDTA remains insoluble). Any suggestions?

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