Re: unstaining and restaining one level

From:Melissa Jensen

The reason for unstaining one section is because the tissue block has been
cut through,past the affected area,that the Pathologist needs a special
stain on. The pathologist also needs the  H&E to verify his original
findings.So I had one slide 3 levels and nothing else..After removing the
coverslip,I decolorized the bottom section.I proceeded with the afb stain
using a pipette to dispense reagent only on that section and also stained
the control the same way..It worked well.I was concerned about the H&E
stained tissue drying out.After thinking about it..I decided it shouldn't be
a problem.Slides have been successfully restored and remounted in the past
when a huge air bubble was found years later after being pulled from the
files..A co worker was uncomfortable with the idea of the tissue [H&E] being
left to the air.So I said I would post the question to the experts.[ps..the
stain worked well and the H&E look great!]
----- Original Message -----
From: "Gayle Callis" 
To: "Melissa Jensen" 
Sent: Thursday, September 19, 2002 11:30 AM
Subject: Re: unstaining and restaining one level

> I think I would do a separate section, new on a separate slide for any
> special stain, you also need to run a positive control slide for that
> anyway. In fact, you can do positive control and unknown on the same slide
> as long as you know which one is which, or use Erie Plus charge control
> slides, separations already done.
> Reasoning: if you destain an H&E section using acids, etc - BEWARE - you
> could alter HOW the special stain works by removal of this stain.
> How would you know if H&E sections are contaminated?? with a special
> ruining those sections also.
>  At 06:13 PM 9/18/02 -0500, you wrote:
> >       Discussion from work.....Could use your  views. &E level [3 levels
> >on  slide]for a special stain...Do you do anyhing to proctect the
> >sections,that were  not stained,from drying out,while you proceed to
> >the bottom section for a  special stain?     Thanks  Much!!!!!
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> email:

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