Re: protein gene product 9.5

From:Andi Kappeler

Hi Annette

We use mo-a-PGP 9.5, clone 13C4, Biogenesis 7863-2004 or Biomeda U3231 (dil.
1:500 and 1:100, respectively). HIER required (citrate buffer pH 6.0,
microwave) for FFPE tissue (3-5 um sections), used on various tissue types;
regular 3-step IHC (ABC, LSAB) on slides, no floating sections. Polyclonal
(rb) PGP 9.5 Abs available e.g. from Dako (no personal experience with this
one). Good luck!

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message -----
From: "Featherstone, Annette" 
Sent: Thursday, September 26, 2002 7:32 PM
Subject: protein gene product 9.5

> Is anyone working with Protein Gene Product 9.5?  What type of tissue are
> you using? Is it a 50u section? Do you float it in antibody?  Does anyone
> have a protocol?
> Thanks
> Annette MLT/HT

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